Fig 1: Knockdown of CH25H facilitates SADS-CoV replication. (A,B) IPI-2I cells were transfected with siRNAs and NC. Cell samples were harvested at 48 hpt. CH25H protein (A) and mRNA (B) were then examined by Western blotting and qRT-PCR. (C–E) IPI-2I cells were transfected with siRNAs for 48 h, followed by infection with SADS-CoV (MOI 0.1). At 24 hpi, cell samples were collected to determine SADS-CoV N protein expression via Western blotting (C) and qRT-PCR (D), and viral titers were detected by TCID50 assay from supernatant (E). Means and SD (error bars) of three independent experiments are indicated. p values were calculated using two-tailed unpaired Student’s t-test.